14个灵芝菌株的酯酶同工酶分析

Esterase Isozyme Analysis of 14 Strains of Ganoderma

对14个灵芝菌株进行拮抗试验和酯酶同工酶聚丙烯酰胺凝胶电泳聚类分析,实验共检出迁移率不同的谱带18条,在50%相似水平上聚合为5类,第1类包括仙芝、大仙823、南韩、园芝6号、甜芝、韩芝;第2类包括赤芝10号、赤芝12号、赤芝、日本红芝;第3类包括紫芝和黑芝;第4类为血芝;第5类为白芝。聚类分析结果与形态观察及拮抗试验结果基本一致。实验还说明酯酶同工酶可作为灵芝良种选育的一种有效标记方法,在菌种鉴定、保藏和管理等方面具有重要作用。

Antagonism, esterase isozyme ployacrylamide gel electrophoretic （EST-PAGE） and cluster analysis was used in the study of 14 strains of Ganoderma. The results showed that there were 18 bands of EST with different Rf. Cluster analysis at 50% similarity level showed that all strains could be clustered into five groups, the first included Xian zhi, Da xian 823, Nan han, Yuan zhi 6, Tian zhi, Han zhi; the second included Chi zhi 10, Chi zhi 12, Chi zhi, Ri ben hong zhi; the third included Zi zhi, Hei zhi; the fourth included Xue zhi; the fifth included Bai zhi. And this result was similar to the result of antagonism and morphological characters. Because of its stablity, the technique of EST can be used for identification of Gamoderma.