固定化Ralstonia metallidurans CH34降解苯酚的研究

Study on Degrading Phenol by Immobilized Ralstonia metallidurans CH34

将既能耐抗重金属又能降解苯酚的细菌Ralstonia m etalliduransCH34固定化以提高其降酚效率。首先通过正交实验,得到了固定化该菌种的最优制备条件,然后对固定化细胞的降酚效果进行了研究。结果表明,固定化R.m etalliduransCH34的降酚效果明显优于游离细胞;抗重金属毒性方面也有较大提高;在加入额外碳源(甲苯,柠檬酸)情况下,固定化R.m etalliduransCH34进行苯酚降解时所受影响明显要小于游离态菌。

The subject of this study is to improve the ability of Ralstonia metallidurans CH34, a phenol-degrading strain with anti-heavy metal feature by immobilization. The optimal conditions for immobilizing bacterial cells were obtained with cross act experiment. The phenol-degrading efficiency of immobilized Ralstonia metallidurans CH34 was investigated. Results show that phenol-degrading efficiency of immobilized cells is obviously superior to that of the free cells ; The ability of its resistance to heavy metal have been greatly improved ; When additional carbon sources were added in the simulated waste water, the immobilized R. metallidurans CH34 were affected less than the free cells.