新生儿缺氧缺血性脑病细胞因子与细胞免疫的变化

Study on the relationship between the neonates with hypoxic ischemic encephalopathy and cellular immune function

目的研究新生儿缺氧缺血性脑病(HIE)患儿白介素(IL)1β、IL10水平及外周血T细胞亚群的变化,探讨免疫学变化在HIE发病中的意义。方法应用ELISA法动态检测HIE患儿及足月正常新生儿出生后第1、3、7天血清IL1β、IL10水平。以Ficoll常规分离外周血单个核细胞,用免疫组化法检测HIE患儿及足月正常新生儿生后第1、3、7天CD+3、CD+4、CD+8、CD+4/CD+8细胞百分率。结果HIE患儿血清IL1β、IL10水平均显著高于正常对照组(P<0.05,P<0.01)且与HIE严重程度有关,HIE患儿外周血CD+3、CD+4、CD+8细胞数量与对照组相比差异有显著性(P<0.01),在不同程度HIE患儿中,以重度HIE患儿细胞免疫功能异常最明显。结论HIE患儿存在一定程度的细胞免疫功能紊乱。IL1β、IL10和T细胞亚群参与HIE的病理生理过程。

Objective To study the changes of interlukin-1β（IL-1β）,interlukin-10 （IL-10） levels and T cell subsets in neonates with hypoxic ischemic encephalopathy （HIE） and its role in pathogenesis of HIE. Methods The levels of IL-1β, IL-10 in serum of the newborns with HIE and normal controls were dynamically detected by ELISA in the first, third, seventh day after birth. The peripheral blood mononuclear cells of newborns with HIE and normal controls were isolated by routine Ficoll-Hypaque, and the levels of CD3^＋, CD4^＋ , CD8^＋ were detected by biotin-streptavidin on spot of the first, thind and seventh day. Results The levels of IL-1β, IL-10 were higher in serum of newborns with HIE than those of normal controls （P〈0.05, P〈0.01） and were associated with severity of HIE. The levels of CD3^＋, CD4^＋ , CD8^＋ in the peripheral blood were higher in serum of newborns with HIE than those of normal controls （P〈0.01）. There was the most abnormal cellular immune function in newborns with severe HIE. Conclusion The certain disorder of cellular immune function can be existent in newborns with HIE. IL-1β, IL-10 and T cell subsets can play a role in pathogenic course of HIE.