摘要
目的: 探讨慢性氟中毒相关基因对患者蛋白质合成的影响.方法:利用cDNA基因芯片技术筛选氟中毒患者和正常对照者外周血淋巴细胞差异表达基因,结果输入Pathway3.0分析软件进行数据处理,用T-test检验初筛,Ratio值进一步筛选,在GenBank进行同源性比较后确认筛选基因.结果:轻度氟中毒组和重度氟中毒组分别与对照组比较,有2个基因表达同时出现显著上调(Ratio值为2.0462～2.9936);有14个基因表达同时出现显著下调(Ratio值为0.0918～0.5034),其中包括与蛋白质合成有关的3个基因:线粒体翻译起始因子2(MTIF2)、单链DNA结合蛋白(PURA)和细胞毒颗粒相关蛋白(TIA1).结论:高氟可以对人体基因表达水平产生一定影响,尤其是MTIF2、PURA和TIA1基因的持续显著下调对基因转录、翻译等蛋白合成过程将产生一定的影响.
Objective： To study effect on protein synthesis by related genes of fluorosis. Methods： To screen differentially expressed genes expression in peripheral blood lymphocytes between chronic fluorosis groups and control group used gene chip technique. The data were analysed with Pathway 3.0. Differentially expressed genes were screened by T - test and Ratio methods, they were identified after BLAST in GenBank. Results： In slight and severe groups compared with control group, there were 2 genes up - regulation （ Ratio： 2.0462～2.9936）;14 genes down- regulation（ Ratio： 0. 0918- 0. 5034 ）in which MTIF2 ,PUPA and TIA1 had close correlation with protein synthesis process. Conclusions：Gene expression on humanblood lyrnphocytes could be effected by excessive fluoride, Especially, persistent and marked down- regulation of MTIF2 ,PUPA： and TIAt genes will have effect on transcription and translation of protein synthesis.
出处
《微量元素与健康研究》
CAS
2005年第4期1-4,共4页
Studies of Trace Elements and Health
基金
国家自然科学基金资助项目(30160079)
关键词
氟中毒
基因芯片
基因表达
蛋白合成
fluorosis
gene chip
gene expression
protein synthesis
