花椒、高良姜挥发油体外抗真菌活性研究

Study on Antifungal Action of Volatile Oil from Pericarpium Zanthoxyli and Rhizoma Alpinae Officinarum in Vitro

目的 研究花椒挥发油、高良姜挥发油对16株皮肤癣茵的体外抗真菌活性。方法 水蒸气蒸馏法提 取精油,采用培养基药物浓度稀释法对两种挥发油进行抗真菌实验。结果 高良姜挥发油对16株皮肤癣菌最小抑 茵浓度(MIC)为0．78-6．25 μL／mL,最低杀菌浓度(MFC)为3．13-50μL／mL。花椒挥发油除了对上海红色毛癣菌 株,石膏样毛癣菌株及武汉猴毛癣菌株三种菌株MIC为1．56μL／mL,对其余菌株MIC均在25-50μL／mL范围内,MFC 结果同抑菌实验。结论 高良姜挥发油有较强的抑真菌活性,花椒挥发油抗真菌活性次之。

Objective To study antifungal action in vitro of volatile oil of Pericarpium Zanthoxyli and Rhizoma Alpinae Officinarum. Methods The minimum inhibitory concentration of both volatile oils against 16 strains dermatophytes were determined by media dilution method. Results All the strains were susceptible to oil of Rhizoma Alpinae Officinarum, their MIC ranged 0.78～6.25μL/mL and MFC 3.13～50 ~、μL/mL. Apart from Trichophyton rubrum （Shanghai strain）, Trichophyton gypserum （Shanghai strain） and Pinoyella simii （Wuhan strain） （MIC 1.56 μL/mL）, MIC of Pericarpium Zanthoxyli against other strains were 25～50 μL/mL, MFC value were similar to its MIC. Conclusion Volatile oil of Rhizoma Alpinae Officinarum showed strong antifungai effect in vitro and oil of Pericarpium Zanthoxyli is lower.