血清HA、CG、C-Ⅳ联检对肝硬化的诊断价值

Diagnostic Value of Combined Determination of Serum HA, CG and C-Ⅳ Levels in Patients with Hepatic Cirrhosis

目的:探讨血清中透明质酸(HA)、甘胆酸(CG)和Ⅳ型胶原(C-Ⅳ)定量检测用于早期肝硬化诊断标志的价值。方法:采用放射免疫分析和固相酶联免疫法检测46例急性肝炎患者、45例慢性肝炎患者、74例肝硬化患者及58例健康人血清中HA、CG、C-Ⅳ的含量。均数比较采用t检验。结果:肝硬化组血清中HA、CG和C-Ⅳ的含量均显著高于急性肝炎组、慢性肝炎组和对照组(P<0.05),且血清中HA、CG和C-Ⅳ的平均水平与肝硬化严重程度呈正相关。结论:患者血清中HA、CG和C-Ⅳ含量的进行性升高可以作为早期诊断肝硬化的有用指标。

Objective To investigate the value of combined determination of levels of serum hyaluronic acid （HA） ,cholyglycine （CG） and type 1V collagen （ C-Ⅳ ） for the early diagnosis of cirrhosis of liver. Methods Serum levels of HA, CG （ with RIA） and C-Ⅳ （ with solid - phase ELISA） were. measured in 46 patients with acute hepatitis, 45 patients with chronic hepatitis, 74 patients with cirrhosis of liver as well as in 58 controls. Results The serum levels of HA, CG and C-Ⅳ in patietns with cirrhosis of liver were significantly higher than those in patients with acute hepatitis, patients with chronic hepatitis and controls （P 〈0.01 ）. Levels of these markers were positively correlated with the severity of the disease as expressed by the child - pugh grading. Conclusion The progressive rising of the serum levels of HA, CG and C-Ⅳ would indicate possible development of early cirrhosis.