AIDS患者外周血淋巴细胞表面CCR5、CXCR4表达情况分析

Coreceptors CCR5 and CXCR4 Expressions on Peripheral Blood T Lymphocytes in HIV/AIDS Patients

目的:研究人免疫缺陷病毒/获得性免疫缺陷综合征(H IV/AIDS)患者淋巴细胞表面第二受体CCR5、CX-CR4的表达情况,分析其临床意义。方法:收集30例H IV/AIDS患者及16例健康对照的抗凝全血,用流式细胞仪检测第二受体CCR5、CXCR4的表达情况,并分析第二受体表达与CD4+T细胞绝对值及T细胞活化(HLA-DR+CD38+)的相关性。结果:AIDS组CD8+T细胞表面CCR5表达明显低于健康对照(P<0.01);AIDS组CD4+T、CD8+T细胞表面CXCR4表达低于健康对照(P<0.01)。H IV/AIDS患者CD4+T、CD8+T细胞表面CCR5、CXCR4的表达与T细胞活化(HLA-DR+)水平明显正相关(P<0.05)。结论:H IV感染者第二受体CCR5、CXCR4的表达与机体对H IV的免疫反应及疾病进展密切相关。

Objective： To investigate the coreceptors CCR5 and CXCR4 expressions on the peripheral blood T lymphocytes in HIV/AIDS patients, with an analysis of their clinical value. Methods ： Thirty HIV/AIDS patients and 16 normal controls were selected and flow-cytometry was used to detect the coreceptors CCR5 and CXCR4 expressions in whole blood samples taken from the patients and controls. An analysis was made of the correlation of the coreceptor expression with CD4 T cell counts and the activation of CD4^＋/ CD8^＋ T cells （ HLA-DR^＋/CD38^＋ ）. Results ： The CCR5 expression on CD8^＋ lymphocytes and the CXCR4 expression on the CD4^＋ / CD8^＋ T lymphoeytes of the HIV/AIDS group were higher than those of the normal controls（ P 〈0. O1 ）. The CCR5 and CXCR4 expressions on the CD4^＋/CD8^＋T lymphoeytes of the HIV/AIDS patients was significantly correlated with the activation of CD4^＋/CD8^＋T cells（ HLA-DR^＋/CD38^＋ ） （ P 〈 O. 05 ）. Conclusion ： The expressions of CCR5 and CXCR4 are significantly correlated with the immune system reaction to HIV and the disease progression in HIV infected patients.