兴安白头翁根茎的化学成分研究

Chemical constituents from rhizome of Pulsatilla dahurica

目的:研究毛茛科白头翁属植物兴安白头翁Pulsatilladahurica根茎中的化学成分。方法:利用硅胶柱、大孔树脂柱、高效液相制备色谱分离纯化,并根据理化性质和波谱数据进行结构鉴定。结果:从兴安白头翁根茎中得到6个化合物,分别鉴定为长春藤皂苷元(hederagenin,Ⅰ),长春藤皂苷元3-O-α-L-吡喃阿拉伯糖苷(hederagenin3OαLarabinopyranoside,Ⅱ),长春藤皂苷元3-O-β-D-吡喃葡萄糖(1→2)-α--L吡喃阿拉伯糖苷[hederagenin3OβDglucopyranosyl(1→2)α-Larabinopyranoside,Ⅲ],长春藤皂苷元3-O-β-D-吡喃葡萄糖(1→2)[βD吡喃葡萄糖(1→4)]αL吡喃阿拉伯糖苷{hederagenin3OβDglucopyranosyl(1→2)[β-Dglucopyranosyl(1→4)]α-Larabinopyranoside,Ⅳ},β谷甾醇(βsitosterol,Ⅴ),胡萝卜苷(daucosterol,Ⅵ)。结论:化合物Ⅰ～Ⅵ均为首次从该植物中分离得到。

Objective： To study the chemical constituents from rhizome of Pulsatilla dahurica. Method： The constituents were isolated and purified by various chromatographic methods. All compounds were identified on the basis of spectral analysis and physieo-ehemieal characters. Result： Six compounds were isolated from the 70% alcohol extract of the rhizome identified as hederagenin （Ⅰ）, hederagenin 3-O-α-L-arabinopyranoside （Ⅱ）, hederagenin 3-O-β-D-glucopyranosyl（1→2）-α-L-arabinopyranoside（Ⅲ）, hederagenin 3-O-β- D-glueopyranosyl（1→2）[β-D-glueopyranosyl（1→4）]-α-L-arabinopyranoside （Ⅳ）, β-sitosterol（Ⅴ） and daucosterol（Ⅵ）, respectively. Conclusion： Compounds Ⅰ-Ⅵ were isolated from this plant for the first time.