贯叶金丝桃总黄酮对小鼠抑郁症模型的实验研究

Experimental study of the total flavonoid in Hypericum perforatum on depression

目的:观察贯叶金丝桃总黄酮的药理作用。方法:通过皮下注射利血平诱导小鼠抑郁症模型,测定药物对利血平效应及单胺类递质含量的影响;此外,测定药物对小鼠脑内单胺氧化酶(MAO)活性、小鼠行为绝望及获得性无助、睡眠等的影响。结果:贯叶金丝桃总黄酮可降低MAO活性,对抗利血平致小鼠抑郁症模型,明显抑制利血平效应引起的小鼠眼睑下垂及自主活动减少,显著增加脑内5羟色胺(5HT)、去甲肾上腺素(NE)的含量,可使原来不会引起异常反应的5羟色胺酸(5HTP)剂量引起小鼠产生震颤反应;此外,明显对抗小鼠行为绝望及获得性无助,延长小鼠睡眠时间。结论:贯叶金丝桃总黄酮对抑郁症有明确的治疗作用。

Objective： To investigate pharmacological effects of the total flavonoid in Hypericum perforatum on depression. Method： Experimental depression was induced by subcutaneous injection of reserpine in mice. The concentration of monoamine transmitters including 5-HT and NE, the activity of monoamine oxidase （MAO） in brain and reserpine-induced symptoms of depression, such as ptosis, attenuation of autonomous activity, behavioral despair, acquired helplessness and sleep, were measured respectively to evaluate the effects of the total flavonoid in H. perforatum on the depression. Result： The total flavonoid in H. perforaturn significantly decreased the activity of MAO, inhibited the ptosis and the attenuation of autonomous behavior induced by reserpine respectively. The levels of 5-HT and NE were also attenuated by the total flavonoid in H. perforatum remarkably. In addition, the total flavonoid in H. perforatum was shown to inhibit behavioral despair and acquired helplessness and to prolong the sleep time in the mice. Following the treatment with the total flavonoid in H.perforatum, 5-THP,at the dosage without any side-effects, caused the tremble in the mice. Conclusion： The results indicate that total flavonoid in H. perforatum can significantly inhibit the depression.