不同自体脾组织移植量抗肺炎球菌感染的实验研究

Experimental study on various amount of autotransplanted spelenic tissue for protection against pneumococcal infection

随着人们对脾脏各种功能的认识以及脾切除后严重感染和心血管疾病的发生.脾损伤后采用各种保脾术越来越多。当严重脾损伤无法保留脾脏时,很多学者仍主张将自体脾组织再移植,但移植后再生的脾组织结构及功能怎样?能否防止发生“OPSI”?尤其是对脾组织的临界移植量问题,无论在临床或是动物实验方面都存在较大争议。本实验采用大鼠进行不同量脾组织网膜内移植,然后进行抗感染能力和实验研究,试图阐明移植脾组织的抗感染作用。移植多少脾组织才是抗感染的理想量,从而为临床处理严重脾损伤提供理论和实践依据。

This study presented the anti -infection capbility and critical amount of autotrans-planted splenic tissue- 360 Wistar rats were divided into six groups- sham operation, total splenectomy, 25, 40, 60 and 80% omental pouch auto - transplantation. After 6 months the anti -infection indexes were measured, and histological architecture was dynamically examined.The results showed: the implanted splenic tissue through oecrosis, regeneration, growth phases, was similar to the nomal spleen? except a few specialcbaracjers were different from the nomal. The autotranspl anten splenic tissue weight neither regenerated to the nomal,nor to the originally implanted amount (The regeneratde splenic weght was significantly correlated to the original implanted. Both the number and area of the splenic nodules in the implantde animals decreased significangly. Among the implanted groups, difference was insignificant, but comparing with the 25% the aren of splenic nodules in 60,-80% group distinctly decreased. After intra -trachea challenge with pneu-mococi (Pn), the survival rate of sham operation and 80% groups were significantly higher than that of the spllenectomy group. PFn. phagcoyte activty in blcod, Pn clearance from the blcodstream of splenectomy group markedly reduced compsred with sham operation, but sll the implanted groups were approximately nomal. After splenectomy, the liver uptake ratd of 3H -TdR labeiled live Pn decreased significantly, the lung one lightly decreased. The implanted splenic tissue one significantly decreased, conpared with nomai spleen.It suggestg that implanted spieen has sbility to protect against infection, amount of implanted splenic tissue must be adequate, autotransplantation of 80% of the spleen is the optimum amount.