PCR技术在鉴定阳性重组子中的应用

Application of PCR technique in the screening of positive recombinants

介绍了采用 ＰＣＲ技术在人粒细胞集落刺激因子（ｈＧ－ＣＳＦ）、人促红细胞生成素（ ｈＥＰＯ）及中国人 ｙ一干扰素（ ｈＩＦＮ－γ） ＣＤＮＡ重组质粒构建过程中快速鉴定阳性重组子的方法。经 ＰＣＲ扩增鉴定为阳性的重组子，提取质粒 ＤＮＡ经重组位点相应的ＤＮＡ内切酶双酶切鉴定表明，全部含有插入片段。

A method for rapid screening of positive recombinants in the construction of hG-CSF, hEPO and hIFN-γ recombinant plasmids by PCR technique is established. All PCR ampliflcation positive recombinants included the inserted foreign genes as verified through corresponding DNA restrictive endonuclease digestion.