骨髓增生异常综合征和急非淋白血病中癌基因及抑癌基因扩增的研究

Studies on the amplification of c-myc, N-ras oncogenes and p53 tumor suppressor gene in myelodysplastic syndrome and acute nonlymphoblastic leukemia

应用核酸杂交技术（ｄｏｔ－ｂｌｏｔ）分析了癌基因。－ｍｙｃ、 Ｎ－ｒａｓ和抑癌基因 ｐ５３在骨髓增生异常综合征（ＭＤＳ）和急非淋白血病（ＡＮＬＬ）中的扩增情况。结果发现，有 ３ 例（３３％）的 ＭＤＳ存在 ｐ５３抑癌基因的 ２倍扩增，本实验中 ｐ５３抑癌基因的扩增发生在 ＭＤＳ的较晚期阶段； １例 ＡＮＬＬ中存在癌基因 ｃ－ｍｙｃ和抑癌基因 ｐ５３的共扩增，且发现扩增与 ＭＤＳ的分型及 ＡＮＬＬ的临床病情有一定的相关性。

Using dot-blot hybridization, the authors studied the amplification of c-myc, N-ras oncogenes and p53 tumor suppressor gene in marrow specimens from 9 cases of myelodysplastic syndrome(MDS), 11 cases of acute nonlymphoblastic leukemia(ANLL), 9 cases of ANLL-CR and 11 cases of other blood diseases. p53 tumor suppressor gene was found to be amplified in 3 MDS, 3 ANLL and I ANLL-CR patients. c-myc oncogene was found to be amplified in 18. 2 percent of ANLL patients. None of the MDS patients was found to have amplified c-myc oncogene. Furthermore, none of the patients had amplified N-ras oncogene. Relating these results with clinical features, it was found that the amplification of p53 tumor suppressor gene took place at a later stage of MDS.