接种烟草花叶病毒后不同抗性番茄品系叶片可溶性蛋白组成的变化

CHANGES IN SOLUBLE TOMATO LEAF PROTEIN CONSTITUTION OF RESISTANT AND SUSCEPTIBLE LINE AFTER INOCULATION WITH TOBACCO MOSAIC VIRUS

用聚丙烯酰胺凝胶电泳(PAGE)分析了不同抗性番茄等基因系GCR—267和GCR—267的叶片可溶性蛋白组成在接种TMV番茄O株后的变化。实验发现敏感品系GCR—26的叶片可溶性蛋白的组成,与健株相比较,TMV侵染株发生显著的变化。接种后7天,除一部分蛋白质含量减少外,还有四种为健株及抗性品系TMV接种株所没有的新蛋白质产生,它们在10%凝胶上的迁移率分别为Rf0.31、0.33、0.39和0.45,其中Rf0.38和Rf0.39两种蛋白质含量极大。接种后10天Rf0.31和Rf0.45两带趋于消失,但Rf0.33和0.39两带继续积累。经电泳和免疫双扩散试验证明Rf0.33蛋白带是TMV外壳蛋白:Rf0.39蛋白带与TMV外壳蛋白无关。该蛋白也不具有过氧化物酶,多酚氧化酶、酸性磷酸酶、酯酶、苹果酸脱氧酶,葡萄糖-6-磷酸脱氢酶或谷氨酸脱氢酶的催化活性,分子中不含有糖类物质。SDS-PAGE测定其分子量为14,200道尔顿。抗性品系GCR-267的TMV接种株的叶片可溶性蛋白组成未出现象感病品系GCR-26那样的变化。讨论了这种变化与番茄抗、感病性之间的关系。

Gel electrophoretic analysis revealed marked alterations in the pattern of base-extractable proteins from tomato leaves of susceptible line,GCR-26 after inoculation with tobacco mosaic virus(TMV)tomato strain O when compared to the pattern from healthy leaves.Four new proteins,Rf0.31,0.33,0.39,and 0. 45,were present 7 days after infection which is most dramatic in the case of the proteins with the Rf value of 0.33 and 0.39.Besides,adecrease was observed in some proteins.10 days after inoculation protein Rf0.31 and 0.45 were not de-tected,while Rf 0.33 and 0.39 accumulated largely.Protein Rf0.33 was charac-terized as TMV coat protein by coelectrophoresis and double immunodiffusion test.Protein Rf 0.39 is not related to virus coat protein and not a new isozyme of peroxidase,polyphenoloxidase,acid phosphatase,esterase,malate dehydrogenase, glucose-6-phosphate dehydrogenase,or glutamate dehydrogenase.It showed a ne-gative reaction with Schiff′s stain for carbohydrates and only gave one poly-peptide with MW.14200 on SDS-gels.From tomato leaves of resistant line,GCR-267,plants we did not observed the same changes in soluble proteins as from GCR-26 plants.These facts showed that changes in soluble leaf protein constitution could be correlated with the symptom development and virus multiplication in tomato plants.