hG－CSF　cDNA重组逆转录病毒的构建

Construction of hG-CSF cDNA recombinant retrovirus

通过ＤＮＡ重组技术，将不含非编码区的人粒细胞集落刺激因子（ｈＧ－ＣＳＦ）ｃＤＮＡ重组入逆转录病毒质粒ｐＬＸＳＮ中。重组质粒转染ＰＡ３１７细胞后，经Ｇ４１８筛选，抗性克隆细胞培养上清液能成功地感染ＮＩＨ３Ｔ３细胞，使之在筛选培养基中形成典型的Ｇ４１８抗性克隆。该克隆细胞染色体中成功地整合了ｈＧ－ＣＳＦｃＤＮＡ，并且表达了有生物学活性的ｈＧ－ＣＳＦ产物。

Granulocyte colony-stimulating factor(hG-CSF)cDNA without non-coding regionwas recombined with retrovirus vector pLXSN through DNA recombinant techniques. After thisrecombinant plasmid was transfected into retrovirus packaging cell line PA317, G418 resistantclones could produce defective G-CSF cDNA recombinant retrovirus successfully which could in-fect NIH 3T3 target cells and make them form typical resistant clones in G418 selective medium.In the genome of these infected target cells,hG-CSF cDNA was successfully integrated and ex-pressed.