水貂肠炎病毒疫苗效检指标及实验动物模型研究

Studies on Indexes and Laboratory AnimalModels for Evaluating the Immune Efficiency of Mink Enteritis Parvovirus Vaccines

测定了水貂肠炎病毒无血清细胞培养灭活矿油疫苗和铝胶疫苗免疫接种水貂、豚鼠和家兔的血清ＨＩ和ＳＮ抗体消长规律，证明豚鼠、家兔或水貂接种疫苗后１１～４０ｄ的血清ＨＩ抗体水平是检定ＭＥＶ矿油疫苗或铝胶疫苗对水貂的体液免疫力和免疫保护率的经济、简便和可靠的指标，建立了ＭＥＶ疫苗免疫效力的检定指标及其实验动物模型。

The change laws of the immune serum haemagglutination inhibition(Hl)and serum-neutralizing(SN)an-tibodies levels for minks, guinea pigs and rabbits following inoculation with the inactivated vaccines fromserum-free cell cultures of mink enteritis parvovirus(MEV)with mineral oil or Al(OH )_3 gel adjuvant havebeen determined by the tests by statistical analyses.It has been proved by statistical analyses that the levels ofHI antibody response of guinea pigs, rabbits or minks determined during 11～40 days postvaccination(P.V.)were reliable indexes for monitoring the humoral immune efficiency and immune protective rate of theMEV vaccine with mineral oil or Al(OH )_3 gel adjuvant for minks,thus providing some economic,simple andscientific new indexes and laboratory animal models for monitoring the immune efficiency of MEV vaccines inlarge scale. All these have been extensively used in productive practice in the last several years.