水稻三系及其杂种F_1的叶绿体同工酶研究

Studies on Isozymes of Chloroplasts in Three Lines and Hybrid F_1 in Rice

以二叶期水稻的两个不育系和相应保持系以及由三个恢复系产生的６个组合的杂种Ｆ＿１代共１３个品种的叶绿体为材料，采用聚丙烯酰胺薄层等电聚焦电泳法，分析了酯酶（Ｅｓｔ）、过氧化物酶ＰＯＤ、细胞色素氧化酶Ｃｙｔ的同工酶谱。结果如下：（１）酯酶同工酶出现１９条酶带，基本酶带６条，Ｆ＿１中出现了偏父本杂交型、完全互补型、父本显性型和父本部分显性型四种类型酶谱。（２）过氧化物酶同工酶显示３０余条酶带。Ａ区有５条酶带仅见于少数品种；Ｂ区有基本酶带６条；Ｃ区酶带变化较大。在Ｆ＿１中出现父本显性型、偏父本杂交型和偏父本部分酶带消失型，（３）细胞色素氧化酶同工酶共显示２０余条．Ａ区５条见于少数品种，Ｂ区为基本酶带区，Ｃ区酶带多变，Ｆ＿１中出现父本显性型、偏父本互补型和偏母本互补型三种类型酶谱。

Using the method established recently in our laboratory,we isolated the pure chloroplasts from twogreen leaf seedlings of 2 male sterile lines,and their maintainer lines,3 restorer lines and 6 hybrid F_1 in rice.The isozyme patterns of esterase(Est),peroxidase(POD),cytochrome oxidase(Cyt)in the chloroplasts wereanalyzed with thin-layer pclyacrylamide gel electrofocusing electrophoresis. The results showed:(1)The es-terase showed 19 bands,6 of them were basic. The band zymograms inhybrids F_1 could be divided into fourzymogram patterns:paternal-tending hybrid band pattern,completely complementary band pattern, paternaldominance band pattern and sectionally paternal dominance band pattern.(2)The peroxidase showed morethan 30 bands,they could be divided into three areas. The A area showed 5 bands which could be found onlyin some strains; 6 bands of B area were basic; the bands in C area varied in different strains.(3)The cy-tochrome oxidase showed more than 20 bands,they could be divided into three areas,too. The A area showed5 bands existing only in some strains. B area is basic band area. Different isozymes zymoRrams were shown inCarea.