芹菜中玉米赤霉烯酮的分离与鉴定

Isolation and Identification of Zearalenone from Apium graveoleus

芹菜属于冬性长日植物,它需要经过低温春化阶段才能开花结实,但低温仅是外界条件,还必需通过植物体内部的生理生化变化才能起作用。关于春化作用机理的研究,自 Melchers(1939)提出低温诱导植物形成春化素(vernalin)的假说以后,Purvis等(1953),Highkin(1955)和Tomita(1959,1964)曾自不同植物中分离出能代替低温或促进开花的物质。

A substance identified to be 'zearalenone'was found in overwintering shoot apices of celeryseedlings. It was isolated by TLC and observedunder UV light (254 nm). It has the same R_fvalue with blue fluorescence ps that of the stan-dard zearalenone (a secondary metabolite ofGibberella zeae, mol wt.= 318). HPLC analysisshowed that it has the same retention time (3.01min.) as that of the standard. Their UV spectraare also identical with 3 peaks at 236,274 and316 nm respectively, MS/MS analysis of the cru-de axtract showed that there is a quasimolecu-lar ion of M / Z 319= MH^+, that means the mo-lecular weight of this substance is 318, and itsdaughter spectrum is also identical with that ofthe standard zearalenone., All these indicatethat zearalenone actually occurs in the overwin-tering shoot apices of celery seedlings. It wasalso noticed that zearalenone (and perhaps itsderivatives) which is closely related to vernaliza-tion may occur widely in winter plants. It mayact as a hormonal signal promoting plants to passvernalization period as well as certain necessarystep before flowering. The physiological effectsof zearalenone should be studied further.