PCR－ODN法检测口腔癌中H－ras癌基因第12位密码子G→T突变

DETECTION OF G-T MUTATION AT CODEN 12 OF THE H-RAS ONCOGENE IN ORAL CMCER BY PCR-ODN

应用聚合酶链反应（ＰＣＲ）放大口腔癌组织中Ｈ－ｒａｓ癌基因部分ＤＮＡ顺序，再以特异突变的寡脱氧核苷酸（ＯＤＮ）探针杂交方法检测口腔癌中Ｈ－ｒａｓ癌基因第１２位密码子的Ｇ→Ｔ突变。结果在２１例口腔癌中有６例存在这种突变（２８．５％），而１０例正常门腔粘膜组织中无１例发生突变。提示：Ｈ－ｒａｓ癌基因第１２位密码子→Ｔ突变可能是口腔癌中癌基因激活的方式之一。

he polymeras chain reaction(PCR) was used to amplify the DNA sequence of the Ha- rasoncogene in the tissue of oral cancer, and the G-T mutation at code 12 of the Ha- rasoncogene was detected by hybridization with mutation specific oligondeoxyucleotide probes .The result showed that the G-T mutation rate was 28.5%( 6/21) in human oral cancers, butabsent in 10 normal oral mucosal tissues.This result implicates that G- T mutation atcodon 12 of the Ha-ras oncogene may be one of the oncogene activated mechanisn in oralcancers .