欧当归原生质体培养中体细胞胚胎发生和细胞学变化

SOMATIC EMBRYOGENESIS AND CYTOLOGICAL VARIATION IN PROTOPLAST CULTURE OF LEVISTICUM OFF1CINALE KOCH

从继代培养的欧当归(Levisticum officinale Koch)愈伤组织分离的原生质体在 C81V培养基中培养,得到了较高频率的持续分裂。形成的细胞团转移到固体培养基上后,发展成了白色松软型的愈伤组织。将其转移到附加6-苄氨基嘌呤和吲哚丁酸的 N6培养基上继续培养了2—3个月后,表面出现了浅黄色、颗粒状的胚性愈伤组织。后者在附加吲嗓丁酸和萘乙酸的 MS 培养基上培养,可通过体细胞胚胎发生途径分化出大量小植株。对供体愈伤组织和原生质体再生植株进行了细胞学观察。

Protoplasts were isolated from spongy calli in a well growing state.Protoplasts were in- duced to undergo sustained divisions and to form colonies in the liquid C81V medium supple- mented with 2,4-D and Kinetin.When protoplast derived colonies were transferred onto agar- solidified medium,the spongy,white calli developed.After being subcultured on N6 medium plus 6BA and IBA, the light -yellow, granular embryogenic calliemerged on the protoplast re- generated callus surface.A large number of plantlets were obtained on MS medium with NAA and IBA via somatic embryogenesis Cytological observation on the donor calli used for proto- plast isolation and plantlets regenerated from protoplasts were carried out.Remarkable varia- tion of nucleus morphology and chromosome numbers were observed in donor calli.Howerer, the cytological abnormalities in plantlets regenerated from protoplasts were comparatively less seen.The reason are discussed.