小地老虎雄性附腺细微结构和功能及高温的影响

ULTRASTRUCTURE AND FUNCTION OF THE MALE ACCESSORY GLAND OF THE BLACK CUTWORM AGROTIS YPSILON (ROTTEMBERG) AND THE EFFECT OF HIGH TEMPERATURE

本文通过光镜、电镜和生化分析等方法，研究了小地老虎Ａｇｒｏｔｉｓｙｐｓｉｌｏｎ（Ｒｏｔｔｅｍｂｅｒｇ）雄性附腺的细微结构和功能，结果表明：（１）雄性附腺是一对管状腺，基段粉红色，中段桔红色，端段乳白色。形态分化在蛹前期完成，分泌功能在羽化后４天进入旺盛期；（２）附腺细胞属蛋白质合成型，具有旺盛的合成蛋白质的能力，胞内含有致密的粗面内质网和游离核糖体颗粒，大量的分泌液泡均匀地分布在细胞质中；（３）顶浆分泌和局部分泌是腺体的二种主要分泌方式，前者分泌的颗粒物为糖蛋白性质（ＰＡＳ阳性），后者分泌的网状物为非糖蛋白性质（ＰＡＳ阴性），二者在腺腔内呈有规则的放射状排列；（４）雄性附腺分泌物具有种的特异性，小地老虎、棉铃虫和粘虫等夜蛾科昆虫分泌物的蛋白电泳谱带存在明显的种间差异，高温（３２℃）抑制了雄性附腺分泌某些蛋白质的能力，从而改变精液的成分。

The ultrastructure and function of male accessory glands of theblack cutworm Agrotis ypsilon (Rottemberg) were studied with light and electronmicroscopies and biochemical analysis. The results are summarized as follows:1. The male accessory gland was a pair of tubules each divided into a pink base,an orange middle part and an opalescent terminal part. The morphological differentiation of the gland was completed during early pupal stage, but it performedfull secretory function on the fourth day after emergence.2. The gland cells were of secretory type with high capacity for protein synthesis. There were a lot of rough endoplasmic reticula and free ribosomes and numerous secretory vesicles in the cytoplasm.3. Apocrine and merocrine types. are suggested as the major secretory activities of these gland cells. The granules formed by apocrine secretion are glycopl'oteinin nuture, but the reticulate secretion formed by merocre activity is not glycoprote In.4. The gland's secretions are species specific in the three species of' noctuids,Agrotis ypsilon, Mythimna scparala and HelicoDerpa armigera. High tempelature(32℃) inhibited gland's capacity to secrete proteins.