马尾松针叶中挥发性成分的鉴定及其对马尾松毛虫的触角电位反应

ANALYSIS OF MASSON PINE NEEDLE VOLATILES AND THEIR ELECTROANTENNOGRAM ACTIVITY WITH MASSON PINE CATERPILLAR

马尾松Ｐｉｎｕｓｍａｓｓｏｎｉａｎａ针叶的挥发物由溶剂提取和真空蒸馏制得。用气相色谱和气相色谱－质谱，由挥发物中鉴定出了２０种成分。它们主要是单萜烯、单萜烯醇、单萜烯乙酸酯和倍半萜烯。用正常和剪除触角的雌蛾研究针叶挥发物在马尾松毛虫Ｄｅｎｄｒｏｌｉｍｕｓｐｕｎｃｔｕｔａｓ产卵场所选择中的作用。试验表明正常雌蛾在针叶上的产卵量要比剪除触角的高得多。触角电位（ＥＡＧ）研究表明无论是交配的还是未交配的雌、雄蛾对针叶挥发物都有强烈的ＥＡＧ反应。用制备气相色谱对挥发物分馏并测定各馏分对来交配和交配雌蛾的ＥＡＧ活性。含有某些单萜烯、单萜烯酸及单萜烯乙酸酯的馏分具有较高的ＥＡＧ活性，其中主要为β－蒎烯的馏分能引起最大的ＥＡＧ反应。

Needle volatiles of Masson pine,Pinus massoniana were obtained by solvent extraction and following vacuum distillation.20 Components were identified from the volatiles with GC and GC-MS.They were mainly monoterpenes,monoterpene alcohols,monoterpene acetates and sesquiterpenes. The roleof the needle volatiles in the selection of oviposition site for the masson pinecaterpillar,Dendrolimus punctatus was investigated with normal and antennectomized female moths.The experiments showed that normal moths depositedmuch more eggs on the pine needles than antennectomized ones.Electroantennogram(EAG)study showed that both sexes of unmated and mated mothshad strong EAG responses to the needle volatiles.The volatiles were fractionated by preparative GC and GC fractions were assayed for EAG activitiesto unmated and mated females.EAG activity was found in the fractionscontaining some of monoterpenes,monoterpene alcohols and monoterpeneacetates.The largest EAG response was elicited by the fraction mainly containing β-pinene.