松材线虫和拟松材线虫不同株系酶电泳的研究

Preliminary Comparision of Bursaphelenchus xylophilus and B. mucronatus Utilizing Enzyme Electrophoresis

应用聚丙烯酰胺凝胶电泳技术对来自国内及日本、加拿大、法国、挪威的松材线虫及拟松材线虫株系作酶电泳的初步分析。结果表明，不同株系的苹果酸脱氢酶及纤维素酶酶谱各不相同。超氧化物歧化酶有１条在不同株系中迁移率相同的酶带。松材线虫与拟松材线虫在酯酶谱上差异明显，但培养于裂褶菌、多毛孢和葡萄孢上的来自南京黑松上的同一株系线虫的酯酶谱也显示了明显差异，表明了该酶的不稳定性。谷氨酸草酰乙酸转氨酶是唯一可区分两种线虫的酶，松材线虫只有１条迁移率０．３９的带，拟松材线虫只有１条迁移率０．５１的带，可用于两种线虫的生化鉴定。对可溶性蛋白质及过氧化物酶的分析，未检测到清晰可辨的酶带。

The enzyme electrophoregrams of Bursaphelenchus xylophilus and B. mu-cronatus that were collected from China,Japan,Canada,France and Norway were analysed. The phenotypes of Malate dehydrogenase(MDH)and Cellulase vary among isolates. Super-oxide dismuatase(SOD )shows a similar pattern with just one main band which migratesslowly.Esterase(Est)is different between B. xylophilus and B. mucronatus but the pheno- type of an isolate from Pinus thunbergii in Nanjing,China shows great difference within theisolate after being cultured on Schizophyllum sp. ,Pestalotis sp.,and Botrytis cineria,sepa-rately. Peroxidase and soluble proteins were also analysed but gave less clear results. Gluta-mate-oxaloacetate transaminase(GOT)isozyme patterns,however,can be used for differ-enting between B. xylophilus and B. mucronatus. Both nematodes have only one band whichis identical introspecies and different interspecies on the basis of Rf.This result coincideswith that of Kiyohara and Bolla's(1990).