喉部肿瘤AgNOR定量研究

Quantitative study of AgNOR in laryngeal neoplasms

应用银染技术对４４例喉癌、７例喉乳头状瘤、８例喉正常粘膜的石蜡切片行核仁组成区相关嗜银蛋白（ＡｓＮＯＲ）含量分析，结果表明：喉癌、喉乳头状瘤、喉正常粘膜之间ＡｇＮＯＲ含量均有显著性差异；ＡｇＮＯＲ含量与喉癌组织学分级、临床分期相关；分析有随访资料的３０例喉癌患者ＡｇＮＯＲ含量，发现术后３年内死亡的１３例和仍存活的１７例病人癌组织中ＡｇＮＯＲ含量有极显著性差异。提示：ＡｇＮＯＲ含量可作为喉良恶性肿瘤鉴别、喉癌分级、分期及预后估计的客观指标。

In this article, the contents of argyrophilic protein of nucleolar organizer regions(AgNOR)were studied in paraffin sections including 44 squamous cell carcinomas of larynx(LC),7 laryngeal papillomas(LP),and 8 normal mucoma(LN).The results showed that average numbers of AgNOR per nucleus have significant differences among each group.It was also found that the mean numbers of AgNOR was correlated significantly with staging and histological grading of LC.During the follow-up of three years,it was noticed that the mean AgNOR,counts of 13 in LC patients who had been dead during the next three years following operations were remarkably higher than those in 17 patients who are still survival.The results indicated that AgNOR count may be a new objective index for monitoring dysplastic laryngeal mucosa,in distinguishing benign and malignant laryngeal neoplasms,assessing the degree of differentiation,and prediction of tumor spread and prognosis of squamous cell carcinomas.