全血2，3－二磷酸甘油酸比色测定法

Colorimetric determination of 2,3-diphosphoglyceric Acid in blood by phosphoglycerate mutase

本文介绍了用磷酸甘油酸变位酶测定２，３－二磷酸甘油酸的比色法。探讨了该法的最适反应条件，最适ｐＨ７．５～７．６。线性范围可达６．０ｍｍｏｌ／Ｌ。三份不同浓度标本批内ＣＶ１．１％～２．１％，批间ＣＶ２．５％～３．９％。６０例南京地区健康成人２．３－二磷酸甘油酸含量为１．６～２．７ｍｍｏｌ／Ｌ，平均回收率９４％，与Ｓｉｇｍａ试剂盒比较测定结果无显著性差异（Ｐ＞０．０５），相关性良好，ｒ＝０．９８２。

In this paper.We reported a colorimetric determination for blood 2,3-Diphosphoglyceric Acid(2,3-DPG) by phosphoglycerate mutase(PGM),We studied optimum conditions of this method and optimum pH was 7.5～7.6.The liner range was up to 6.0 mmol/L. The coefficient of variation of three samples in vary concentration was 1. 1%～2. 1% intr-assay and 2.5%～3.9% inter-assay.The reference value in blood 2, 3-DPG of 60normal adults in Nanjing area was 1. 6～2. 7 mmol/L. The mean recovery rate was 94%. The results estimated by our kits correlated closely with those obtained by Sigma kits and the correlation coefficients was r=0. 982. This method is rapid, simple and accurate. The regent had a long term stability and was easy to buy and was convenient In use.