幽门螺杆菌感染与胃窦内分泌细胞的变化

The Changes of Antral Endocrine Cells in Helicobacter Pylori Infection

收集65例胃窦粘膜活检标本。应用4种内分泌及激素抗体的免疫组化方法,并通过图象分析定量技术显示内分泌细胞的种类及数量。应用Warthin-Starry染色法显示幽门螺杆菌(HP)。结果发现伴有HP感染组(18例)的内分泌细胞的种类及数量均有变化,G细胞数量增多(71.28±12.5/mm^2),D细胞数量减少(5.32±1.42/mm^2),蛙皮素阳性细胞亦增多(12.7±2.6/mm^2)。G/D比值为13.4。不伴HP感染组(47例)G细胞定量为67.65±10.4/mm^2,P>0.05;D细胞为13.65±3.56/mm^2,P<0.05;蛙皮素阳性细胞为5.31±2.32/mm^2,P>0.05。G/D比值为5.05。上述结果表明,幽门螺杆菌感染引起胃泌素升高的原因可能是D细胞的数量减少,分泌胃体抑素较少,使C细胞分泌的抑制因素降低的结果。

65 cases of antral mucosal biopsies were observed by immunohis-tochemical staining using 4 types of antibodies against chromogranin A, gastrin,so-matostatin and bombesin. The positive cells were quantitatively studied by image analyzer. The number of G cells was 71.28/mm2in the group of helicobacter pylori (HP) infection and D cells was 5.32/mm2, bombesin positive cells 8.68/mm2, the ratio of G/D cells was 13.40. In the group without HP infection the number of G cells was 67.65/mm2, D cells was 13.65/mm2,bombesin positive cells 5.31/mm2, the ratio of G/D cells was 5.05. The number of D cells and ratio of G/D cells were significantly different (P<0.05). It suggested that gastrin incresed in patients with HP infection may be duo to the decrease in D cell and somatostatin secreting.