应用聚合酶链反应技术检测肝组织中的HBV－DNA

Detection of hepatitis B virus DNA in liver tissue using the polymerase chain reaction assay

采用聚合酶链反应（ＰＣＲ）技术，对４２例肝活切组织石蜡切片中乙型肝炎病毒（ＨＢＶ）ＤＮＡ进行检测，并与乙肝表面抗原（ＨＢｓＡｇ）的免疫组织化学及血清学检测进行比较，ＨＢＶ－ＰＣＲ阳性率为７３．８％，高于组织及血清ＨＢｓＡｇ阳性率（分别为５９．５％和５０．０％）。３例病理形态呈肝炎改变，而血清ＨＢｓＡｇ（─）的肝组织中有２例检出ＨＢＶ－ＤＮＡ，提示ＰＣＲ的高度敏感性和准确性。８３．３％的门脉性肝硬变和８７．５％的肝细胞癌组织中ＨＢＶ－ＰＣＲ呈阳性，进一步证实了上述两病与ＨＢＶ的关系密切。我们还发现肝细胞淤胆患者ＨＢＶ感染率较高，ＨＢＶ－ＤＮＡ及组织ＨＢｓＡｇ阳性比例各为６／９和４／８。

The hepatitis B virus(HBV)DNA was detected in paraffin embedded liver tissues using the polymerase chain reaction(PCR)technique,and compared with the HBsAg immunocytochemistry and its serology. The results showed that the positive rate of HBV-DNA which was 73.8%was higher than either of the HBsAg positivity which was 59.5% in the liver tissues and 50.0% in sera,Two in three cases with pathomorphological changes of hepatitis but serum HBsAg negative showed HBV-PCR positive in the liver tissues,83.3% of portal cirrhosis and 87.5%of patients with hepatocellular carcinoma showed HBV-PCR positive. It was indicated the close relationship between the two diseases and HBV infection.A high HBV-PCR positive rate of 6/9 cases was also noted in patints with biliary pigmentation in hepatic cells.