全反维甲酸对黑素细胞与瘤细胞粘连性与移动性的影响

Effects of all-trans retinoic acid on melanoma cells adhesion and motility

人表皮黑素细胞经全反维甲酸（ＲＡ）处理后，置于含有牛血清白蛋白、纤维连结蛋白与层粘连蛋白的培养板中检测其粘连性。结果发现经３×１０（－６）ｍｏｌ·Ｌ（－１）的ＲＡ处理的黑素细胞对上述３种蛋白质的粘连性及移动性均被抑制。人和小鼠黑素瘤细胞系经上述浓度ＲＡ处理后。

Human epidermal melanocytes were treated with all-transretinoic acid (RA) and examined for adhesion to bovine serum albumin, flbronectin- and laminin-coated culture dishes.Control and treated cells were also examined for motility into micropore filters coated with the same proteins. Treatment of the cells with 3×10(-6) mol·L(-1) RA for 3～4 days resulted in inhibition of attachment to all three substrates. Decreased attachment was observed within 1.5 hours. Inhibition of attachment was not due to toxicity because differences between control and treated cells disappeared by 18 hours, when most of the cells (approximately 75%) were attached and spread on all three substrates. The same treatment that inhibited adhesion also reduced migration into the interstices of micropore filters coated with the same three proteins. In additional experiments, human and mouse melanoma cell lines were examined in place of normal melanocytes. RA treatment also blocked adhesion and motility of these cells. The malignant melanoma cells were less sensitive to RA than normal melanocytes in the adhesion assay but were equally sensitive in the motility assay. The ability of RA to inhibit melanocyte adhesion and motility as well as melanocyte growth could explain, in part, the capacity of retionoids to modulate melanocyte function in hyperpigemented skin lesions.