胃良、恶性病变组织中核仁组成区的研究

Studies of nucleolar organizer regions in the tissues of gastric benign and malignant lesions

应用核仁组成区染色技术系统地研究了正常胃粘膜、胃粘膜异型增生，早期胃癌及进展期胃癌的核仁组成区（AgNOR）数量和形态变化，发现正常胃粘膜AgNOR计数最低，形态无畸形，随着胃粘膜病变的进展，AgNOR计数逐渐升高，且形态上的畸形程度也随之增加。AgNOR计数与胃癌的浸润深度呈正相关，与胃癌的组织分化程度呈负相关，高AgNOR计数的胃癌易发生淋巴结转移。本研究结果提示，用AgNOR计数法，同时结合AgNOR畸形度，对鉴别中、重度胃粘膜异型增生与早期胃癌有一定帮助，且AgNOR计数有可能作为判断胃癌生物学行为的一个定量性指标。

The quantitative and morphologic changes of nucleolar organizer regions (AgNOR) in the normal gastric mucosa, mucosal dysplasia, early gastric carcinoma and advanced gastric carcinoma were systematically studied by using silver staining of nucleolar organizer regions.It was found in normal mucosa that the mean AgNOR count was lowest and there was no morphologic de formity of AgNOR,and the mean count and morpholgically deformed degree of AgNOR were gradually increased with the progression of gastric mucosal lesions. The mean AgNOR count was positively related to the infiltrative depth of gastric carcinoma and reversely related to the differentiation of gastric carcinoma. The gastric carcinoma with a higher mean AgNOR count was easy to metastasize to lymph node,The results suggest that the method of AgNOR count with the AgNOR deformed degree might be helpful to distinguish early gastric carcinoma from middle or severe dysplasia, and that the AgNOR count could be used as a quantitative indicator for the assessing of the biological behavior of gastric carcinoma.