人血清及初乳中红桂木凝集素结合蛋白的研究

Research on Artocarpus Lingnanensis Lectin Binding Proteins of Hu-man Serum or Colostrum

我们采用Ｇａｌ－Ｓｅｐｈａｒｏｓｅ６Ｂ柱从红桂木种子抽提液中分离出纯化的红桂木凝集素（ＡＬＬ），并制成ＡＬＬ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和柱，从人血清及初乳中分离出ＡＬＬ结合蛋白。免疫学鉴定发现血清中ＡＬＬ结合蛋白是ＩｇＡ、ＩｓＧ，其中以ＩｇＡ为主；初乳中ＡＬＬ结合蛋白是ＳＩｇＡ。研究结果显示，ＡＬＬ－Ｓｅｐｈａｒｏｓｅ４Ｂ可用于从人血清及初乳中快速、大量纯化ＩｇＡ。

Artocarpus Lingnanensis Lectin(ALL)was purified by Gal-Sepharose 6B columnchramotography and prepared for ALL-sepharose 4B affinity column to isolate ALL- bindlng protein fromserum or colostrum, By immunologic identity,it has been found that ALL can bind human serum IgA,IgG(mainly IgA)or colostrum IgA, The results showed that ALL should be a useful tool for the fast preparationof serum IgA or secretory IgA in high yields.