多抗F（ab＇）_2－ELISA测定人血清抗TG－Ab_2及意义

Detection of anti-thyroglobulin idiotypic antibodies in human sera us-ing polyclonal antibody F(ab')_2 ELISA

为了克服单抗测定甲状腺球蛋白独特型抗体（抗ＴＧ－Ａｂ＿２）的局限性，便于常规检测，我们建立了兔抗ＴＧ多克隆抗体的Ｆ（ａｂ＇）＿２－ＥＬＩＳＡ测定人血清中的抗ＴＧ－Ａｂ＿２．结果其抗ＴＧ－Ａｂ＿２阳性率：甲亢（Ｇｒａｖｅ＇３）为９．９％（７／７１），桥本氏甲状腺炎为４４．４％（４／９），甲状腺瘤为１６．７％（３／１８），ＳＬＥ为９．５％（４／４２），类风湿性关节炎为０％（０／３２），正常人为０％（０／３５）。

By using rabbit anti-thyroglobulin polyclonal antibody F(ab')_2,the ELISA of detecting anti-thyroglobulin idiotypic antibody in human sera was established for the convenience of routine test in order toeliminate limitation of using monoclonal antibody. The positive ratio ware 9.9%(7/71)in patients withGrave's disease,44.4%(4/9) Hashimoto' s thyroiditis,16.7%(3/18)thyroadenomata,9.5%(4/42)SLE,none(0/32)rheumatoid arthritis,and none(0/35 ) in normal subjects.