抗盐碱罗布麻DNA导入棉花的研究

Studies on Introducing Salt Resistance DNA of Dogbane into Cotton

利用微注射技术，把抗盐碱罗布麻ＤＮＡ导入鲁棉６号。对其后代经人工配制的盐碱土筛选育成了两个耐盐碱品系９１－１１和９１－１５，在含盐量为０．５１％的滨海盐碱地种植，其皮棉产量分别比受体亲本鲁棉６号增产１９１．７％和２３７．８％。对９１－１１和鲁棉６号在盐胁迫条件下测定其细胞质膜透性和超氧化物歧化酶（ＳＯＤ）活性，结果表明，９１－１１在盐胁迫条件下能够维持较高的ＳＯＤ活性和较稳定的膜透性，而鲁棉６号呈现相反的变化，而且差异显著。据此说明，应用这一技术能够筛选出含有抗性基因的改良品种。

Exogenous dogbane(Apocynum venetum)DNAs with salt resistance were intro-duced into the ovaries of variety Lumian 6 (G. hirsutum L.)after self pollination by injectionmethod.The second generation(D2)were screened for salt tolerance by using saline-alkalisoil,two salt tolerant strains 91-11 and 91-5 were identified.They were planted in the soilwith 0.51%saline-alkali concentration,yield of 91-11 and 91-15 was 191.7%and 273.8%as much as that of Lumian 6.Superoxide dismutase(SOD)activity and cell membrancepermeability were characterised in various organelles of recepient Lumian 6 and 91- withdifferent salt tolerance during salt stress. The results showed that seedlings of cotton exhibitedvery significant differences in both SOD activity and membrane permeability under salt stress,the higher SOD activity and approxmately integral membrance system could be maintained incotton seedling of 91-11,whereas in Lumian 6,an opposite result was obtained.Finally,itis suggested that,using this technique,resistance genes can be screened in varieties.