雷公藤多甙对家兔急性实验性血清病免疫功能的影响

Immunoregulation of Tripterygium Wilfordii Polyglycosidiumon the Acute Experimental Serum Sickness in Rabits

为了探讨雷公藤多甙（ＴＷＰ）对家兔急性实验性血治病（ＡＥＳＳ）的免疫调节作用，我们以家兔ＡＥｓＳ为实验模型，观察ＢＳＡ免疫实验动物后的不同时期ＴＷＰ对家兔外周血Ｔ和Ｂ淋巴细胞增殖的影响、白细胞介素－２（ＩＬ－２）活性的影响及肾脏组织的病理学变化。结果表明：ＴＷＰ明显抑制ＣｏｎＡ诱导Ｔ淋巴细胞的增殖，但对ＬＰＳ诱导的Ｂ淋巴细胞增殖无影响。抑制ＣｏｎＡ诱导Ｔ淋巴细胞产生ＩＬ－２；抑制循环免疫复合物（ＣＩＣ）的形成；减少肾小球内炎性细胞浸润和肾小球内免疫复合物（ＩＣ）沉积。提示：ＴＷＰ可能通过抑制Ｔ淋巴细胞的功能，发挥对家兔ＡＥＳｓ的免疫调节作用，从而减轻ＡＥＳＳ发病进程中肾脏病理损伤的程度。

In order to investigate the effect of tripterygium the wilfordii polyglycosidium(TWP)on theacute experimental serum sickness(AESS)in rabbits,AESS is used as an experimental model,and 24 rabbitswere randomly divided into the experimental group and the control group with 12 in each. In the experimentalgroup,l day before and after the injection of bovine serum albumin(BSA),TWP of 30mg/kg·d was fivenperidonely for 2 weeks. For the control group,only equal amount normal saline instead of TWP. The effect ofTWP on proliferation of PBMC ,activity of interleukin-2(IL-2) and pathological changes in kidneys were in-vest igated.The results showed that TWP could effectively suppress the proliferation of T lymphocytes in-duced by ConA. But conld not supress that of B lymphocytes ind uced by LPS. Moreover,TWP could inhibltthe production of IL-2 in T lymphocytes induced with ConA,and inhibit immune complex depositlon and in-flammatory cell infiltration in the glomeruli.It is suggested that TWP have immunomodulatory effect onAESS by inhibitory function of T lymphocytes,and so it can lessen injury of the kidneys.