油菜花粉变应原的分离纯化及变应原性实验研究

Experimental Studies on Isolation,Purification and characterizationof Brassica Campestris Pollen Allergens

油菜花粉经硫酸铵沉淀，ＳｅｐｈａｄｅｘＧ－２５凝胶过滤，分成组份Ａ和组份Ｂ，前者有明显变应原性。组分Ａ经ＤＥＡＥ纤维素层析，低盐浓度洗脱得组份Ｃ，高盐浓度洗脱得组份Ｄ。前者为主要变应原，１００μｇ／ｍｌ触发肥大细胞，组胺释放率为５５．０％。油菜花粉主要变应原组份Ｃ是一碱性糖蛋白，分子量为３５７１５．６ｕ，含糖串１１．４％，Ｎ－末端为组氨酸。等电聚焦电泳分折，有两种不同等电点区带，为ｐＩ７．７和７．１。

The allergenicity of Brassica campestris pollen components was studied.The purified allergen,component C,was obtained from this pollen by ammonium sulfate precipitation,Sephadex G-25 gel andDEAE cellulose chromatography. The component C is a basic glycoprotein containing 11.4% of carbohydrate. It’s molecular weight is 36715.6u with histidine at N-terminal.Two subfractions with pI 7.7 and pI7.1 wereseparated by ixoelectrofocuxing electrophoresis. It was reflected that the component C had the charge hetro-geneity.The allerglc rat mast cells histamine release rate was of 55.0%as triggering with 100μg ml of com-ponent C. The component C is the main allergen of Brassica campestris pollen.