免疫小鼠GALT部位T淋巴细胞回忆反应观测

The Memory Responses of T Lymphocytes in GALT ofS Typhimurium G30 Infected Mice

以鼠伤寒杆菌减毒株Ｇ３０经口免疫小鼠两次，间隔３或５个月后再刺激，应用间接免疫荧光法检测了小鼠派伊尔氏（ＰＰ）淋巴结、肠系膜淋巴结（ＭＬＮ）及脾脏（ＳＰＬ）中Ｌ３Ｔ４、ＬＹＴ２Ｔ细胞亚群变化并以ＭＴＴ法测定了ＣｏｎＡ诱导的淋巴细胞增殖反应。实验表明：Ｇ３０菌能在ＰＰ与ＭＬＮ中激发Ｌ３Ｔ４亚群及淋转的回忆反应，此外，ＳＰＬ也产生了Ｌ３Ｔ４亚群的记忆应答。提示：Ｇ３０菌确实能诱导细胞免疫应答并形成较强的免疫记忆。

After giving oral primed mice the booster dose of S typhimurium G30 three or five moonths later,L3T4、MLN(Mesenteric lymph nodes)and SPL(Spleen)were detectcd by indirect immunofluorescence and MTT assay respectively. Results showed that significant memory responses of L3T4 subpopulation and proliferation were induced in PP and MLN. The study demonstrated that S.typhimurium G30 could evoke a cell-mediated immune memory responses Indeed.