抗人Clq轻链可变区基因在E．coli中的表达

The Expression of V_kGene of Anti-HumanC1_qMonoclonalAntibodyin E.coli

应用基因工程技术，将抗人Ｃｌｑ轻链可变区基因（ＣｌｑＶ＿Ｌ）由重组质粒ｐＧＥＭ－ＣｌｑＶ＿Ｌ中分离，克隆进表达载体ｐＪＬＡ５０２。该表达载体含ＣＩＴｓ８５７抑制子基因，通过开温诱导法，重组表达子在宿主菌ＨＢ１０１中表达，获得抗人Ｃｌｑ轻链可变区片段，ＳＤＳ－ＰＡＧＥ表明其分子量范围为１２～１３ＫＤ。这为进一步抗人Ｃｌｑ小抗体的表达积累了经验，有利于用于抗原－抗体结构功能的研究。

The light chain variable gene of mouse anti-human Clq monoclonal antibody was isolated fromrecombinant plasmid pGEM-Clq,subsequently cloned into expression plasmid pJLA502 with CITs857 gene,and transformed into E. coli strain HB101.Shifting the culture temperature from 30℃ to 42℃ led to the ex-pression of ClqV_L with a molecular weight of 12～13KD. The result is significant for the furthe constructionof anti-Clq Fv,as well as for the study of structure-function between Clq and anti-Clq antibody.