离心洗脱法在Rb蛋白与细胞分化关系研究中的应用

Application of Centrifugal Elutriation for the Study of Relationship between Rb Protien and Cell Differentiation

离心洗脱技术可分离到ＨＬ６０细胞周期中同步的ＧＯ／Ｇ１期细胞．被视黄酸（ＲＡ）诱导的Ｇ０／Ｇ１细胞可以进行一个正常的细胞周期，Ｒｂ蛋白磷酸化水平随细胞周期而变化．但不能进入第二个细胞周期，细胞开始分化，Ｒｂ蛋白处于低磷酸化水平．Ｒｂ蛋白磷酸化水平的降低，是由于ＲＡ首先诱导细胞分化，使细胞生长停止。

Centrifugal elutriation method have been used to obtain a homogeneous cell population with regard to cell-cycle phases and stage of differentiation. In the presence of retinoic acid, the synchronous G0/G1 HL60 cells could completed first cell -cycle, the phosphorylation of Rb protein varied in the phases of cell-cycle. However the RA induced cells could not entry the second cell-cycle, but started to differentiate, and the Rb protein was observed to be in a state of under-level phosphorylation. The under level or dephosphorylation of Rb protein might be attributed to the induction of cell differentiation by retinoic acid.