培养心肌细胞缺血性损伤及抗损伤措施的定量研究

Quantitative study on ischemic and antiischemic myocardium injury in cultured myocardial cell

本文研究了亚硒酸钠(sodium selenice,Se)和1,6-二磷酸果糖(fructose-1,6-diphosphate,FDP)对乳鼠培养心肌细胞缺血早期影响的形态计量学和定量细胞化学。用图像分析系统(textureanalysis system,TAS)同步动态定量测定了心肌细胞和亚细胞的形态以及细胞化学的改变,并观察了心肌细胞脂质过氧化(lipid peroxidation,LPO)和搏动功能的改变。结果提示:线粒体体积和数目的改变呈高度负相关,当线粒体肿胀超过其自身大小的一倍时,溶解破坏增多;Se和FDP对缺血心肌细胞均有保护作用,但Se对防止线粒体溶解,维持心肌细胞的琥珀酸脱氢酶(succin c dehydrogenase,SDH)活性及降低缺血心肌细胞丙二醛(malondialodehyde,MDA)含量等作用明显强于FDP,而FDP减轻心肌细胞和线粒体肿胀,改善心肌细胞搏动的作用强于Se。

The ischemic myocardium in cultured myocardial cell of newborn rat and treated with Sodium Selenite(Se) and Fructose-1, 6-diphosphate(FDP) were studied comparatively by means of morphometry and quantitative cytochemistry. The area of myocardial cell and mitochondrion and the content of succinic dehydrogenase (SDH) in myocardial cell were quantitated by texture analysis system (TAS). Lipid peroxidation(LPO) and the myocardial cell beat function were observed. The results showed that the changes of the mitochondrion area interrelated with its number remarkabiy. When the mitochondrion area enlarges more than two fold, the destruction of mitochondrion is remarkable. Both Se and FDP can protect the ischemic myocardial cell, but the effects Se on forestalling destruction of mitochondrion, maintaining vitality of SDH and reducing content of malondialodehyde (MDA) are more powerful than FDP, and the effects of FDP on reducing edema of myocardial cell and mitochondrion, improving myocardial cell beat function are better than Se.