山莨菪碱对内毒素与大鼠离体心脏及肝细胞结合的抑制作用

Anisodamine (654-2) decreases endotoxin binding to rat heart and hepatocytes

本实验用含异硫氰荧光素标记的内毒素(FITC内毒素25μg/ml)的灌流液及含内毒素和山莨菪碱(各25μg/ml)的灌流液进行大鼠离体心脏灌流。经荧光分光光度计测定灌流前后灌流液内毒素含量,发现山莨菪碱使内毒素与心肌组织结合率降低47.7%(P<0.02)。分离的大鼠肝细胞与FITC内毒素共同孵育,预先或同时加入山莨菪碱均可明显抑制内毒素与肝细胞结合。本实验说明:山莨菪碱在离体组织器官及细胞水平均可抑制宿主缅胞与内毒素的结合。提示:山莨菪碱在细胞膜水平上与内毒素相拮抗。

Rat heart was perfused on Langendorff apparatus with Krebs-Henseteit buffer which contained Fluorescein Isothiocyanate-Lipopoly-saccharide (FITC-endotoxin, 25μg/ml) with or without 654-2 (25μg/ml), and the amount of endotoxin(ET) in the perfusion solution was measured before and after perfusion using fluorescence spectrometer. It was discovered that 654-2 decreased the binding of ET to myocardium by 47.7%. The difference between the 654-2 treated and the control group was significant (P<0.02). Incubation of isolated rat hepatocytes with FITC-ET in vitro was served as investigated material. The binding rates of ET with hepatocytea preincubated or incubated with 654-2 were both lower than that of the control group which contained no 654-2 in the incubation fluid. 654-2, however, is unable to extract the ET away from the hepatocytes when ET were already binded to the hepatocytes. Results indicate that 654-2 can decrease the binding of ET to tissues and cells in vitro. It is suggested that 654-2 may be potentiated in acting as an effective endotoxin antagonist on the cell membrane.