鸡传染性支气管炎病毒血凝性的研究

STUOV ON THE HEMAGGLUTINATION ACTIVLTY OF AVIAN INFECTIOUS BRONCHIS VIRUS

用中国和澳大利亚分离的Ａ型魏氏梭菌培养液、Ⅰ型卵磷酯酶Ｃ和胰酶处理鸡传染性支气管炎病毒（ＩＢＶ），制备的血凝抗原能凝集鸡红细胞，为鸡传染性支气管炎的诊断提供了方法。对鸡传染性支气管炎Ｍ（４１）、Ｈ（１２０）、Ｈ（５２）、Ｇｒａｙ、Ｃｏｎｎｅｃｔｉｃｕｔ、Ｔ、ＧＩＢＶ等７个毒株进行了血凝性的比较，以Ｈ（１２０）毒株血凝价最高，其次是Ｍ（４１）、ＧＩＢＶ、Ｇｒａｙ，而Ｈ（５２）、Ｃｏｎｎｅｃｔｉｃｕｔ、Ｔ等毒株血凝性低或无血凝性。作者还对影响鸡传染性支气管炎病毒血凝试验的各种因素进行了研究。

Avian infectious bronchitis virus(IBV) was treated with the culture of Clostridium welchii, type A isolated from Australia and China, phospholipese C, type 1 and tryghnase. The modified IBV can aselutinate Chicken RBC, and it provides a good diagnostic method for IBV. Comperation of seven strains IBV for their hemagglutination (HA) activity indicated: the H12o strain had the greatest HA activity, and M41, GIBV, and Gray strains had the moderate, but H52, Connecticut, and T strains had lower or no HA activity. Various factors influencing the HA tests of IBV were also investigated.