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猪体外受精技术中生殖细胞体内外成熟及获能的比较

COMPARISONS BETWEEN MATURATION AND CAPACITATION OF GERM CELLS IN VIVO AND IN VITRO FOR IN VITRO FERTILIZATION OF PIGS
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摘要 猪体内、外成熟卵子与体外获能精子进行受精,其卵裂率分别为43.6%和19.6%,两者差异极显著。体内获能精子与体外成熟卵子进行受精,其受精率和卵裂率分别为55.0%和22.5%,均高于体外获能精子组的水平,但差异都不显著.体内获能精子不能经受常规的精子冷冻程序。 After in vitro fertilization, the cleavage rate of oocytes matured in vivo was higher than that in vitro (43. 6 % vs 19. 6 % ). The fertilization rates of spermatozoa capacitated in vivo and in vitro were 55. 0%, 37. 5 % and the cleavage rates were 22. 5%, 12. 8% respectively. There was no real difference in the two kinds of rates. The capecitated spermatozoa were unable to stand the conventional procedure of sperm freezing.
出处 《南京农业大学学报》 CAS CSCD 北大核心 1995年第4期71-74,共4页 Journal of Nanjing Agricultural University
关键词 精子 获能 成熟 体外受精 pig spermatozoon capacitation maturation in vitro fertilization
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