电镜原位杂交对淡水三角头涡虫中期染色体RNA的研究

STUDY OF RNA IN THE METAPHASE CHROMOSOMES OF Dugesiidae BY IN SITU HYBRIDIZATION

本文用ＲＮａｓｅ－ｇｏｌｄ标记法定位了淡水三角头涡虫中期染色体内ＲＮＡ，发现ＲＮＡ在染色单体切面内部及边缘均有分布。此外，以玉米１８ｓｒＲＮＡ的ｃＤＮＡ和水稻５ｓｒＲＮＡ的ＤＮＡ为探针，经电镜原位杂交后观察了涡虫１８ｓｒＲＮＡ（也包括４５ｓｐｒｅ－ｒＲＮＡ）及５ｓｒＲＮＡ在中期染色单体切面上的分布特点，证明涡虫染色单体边缘及内部ＲＮＡ的两个来源是核仁核糖体ＲＮＡ和核基质内的５ｓｒＲＮＡ。

In the paper, the RNAs in metaphase chromosomes of Dugesiidae are located using RNase-gold labelling. The result shows that RNAs distribute in the inner and border regions of the chromosome sections. By in situ hybridization, the distributional character of 18srRNA (or 45s pre-rRNA) and 5srRNA on Dugesiidae metaphase chromosome sections is also studied under an electron microscope. The outcome indicates that 18srRNA,5srRNA are two among the origins of the inner RNAs and peripheral RNAs of Dugesiidae metaphase chromosomes.