快速提取临床标本中结核杆菌DNA方法的研究

A Study on a Rapid Method for Extraction of Mycobacterium Tuberculous DNA from Sputum and Body Fluids

介绍了二种快速、简便从临床结核性痰标本及体腔液标本中提取DNA的方法,40例结核性标本PCR扩增结果:加热碱裂解法阳性率为67.5%(27/40);溶菌酶法阳性率是60%(24/40).二种方法经统计学处理,PCR阳性率之间无显著性差异(P>0.05).这些方法中,我们省去了传统的酚拙提及蛋白酶K的作用步骤,使提取DNA能在1小时内完成,适用于临床推广.

M. Tuberculous DNA was extracted from sputum and body fluid of pa-tients with tuberculosis by alkali or lysozyme hydrolysis. Of 40 tuberculous specimens, the positive rate revealed by PCR was 67. 5%and 60. 0% respectively. There was no significant difference between these two methods (p>0. 05). The results showed that these two meth-ode without phenol extraction and prothinase K digestion were simple and rapid than the con-ventional method. The whole procedure could be completed within one hour. They are rec-ommended for clinical laboratories.