聚合酶链反应检测泌尿系结核患者尿中结核杆菌

Polymerase Chain Reaction Assay Detected Mycobacterium Tuberculosis in Urine Speciments from Tuberculosis of Urinary System Patients

用聚合酶链反应（ＰＣＲ）技术对３４例泌尿系结核病人的２４小时尿液标本进行结核杆菌染色体中３３６－ｂｐ重复序列ＤＮＡ片段的扩增检测，阳性率为９４．１％。ＰＣＲ检测２４小时尿样中结核杆菌比尿沉淀物涂片法找抗酸杆菌（阳性率５６％）及结核杆菌快速培养法Ｂａｃｔｅｃ培养结核杆菌（阳性率７０．６％）更敏感。我们还用ＰＣＲ、涂片法、Ｂａｃｔｅｃ培养法检测了１０例非泌尿系结核者尿中结核杆菌，阳性结果分别为０、０、３例，ＰＣＲ无假阳性。ＰＣＲ与涂片法、ＰＣＲ与培养法检测尿中结核杆菌两两比较有显著性差异（Ｐ＜０．０１）．且ＰＣＲ耗时短，是检测泌尿系结核患者尿中结核杆菌的最佳方法。

A polymerase chain reaction assay with an amplification of a 336-bp repetitive fragment in the chromosome of Mycobacterium tuberculosis was used to demonstrate M.tuberculosis DNA in 32 of 34 (32/34) urine specimen from patients with tuberculosis of urinary system and it was shown to be more sensitive than Bectec culture (24/34) and acid-fast staining(19/34).(P<0. 01,respectively). The specific DNA fragment can be detected in 100 organisms/ml. The assay is specific for M. Tuberculosis, When chromosomal DNAs from other bacteria were used, the 336-bp band was not synthesized,PCR.Bectec culure and acid-fast stain also were used to detect M. tuberculosis in 10 urine speciments from nontuberculosis of urinary system individuals.The positive results were 0,0, 3 respectively.PCR assay was a sensitive, specific and rapid test for the laboratory diagonosis of tuberculosis of urinary system.