一种有效的肿瘤耐药逆转剂─汉防己甲素逆转白血病耐药的实验研究

An Effective Reversal Agent of Drug Resistance in Tumor Cells──Study on Reversal of Drug Resistance by Tetrandrine in Leukemic Cells

用ＭＴＴ法检测ＤＮＲ及ＨＨＴ对白血病细胞的毒性作用，并观察中药汉防己甲素（ｔｅｔｒａｎｄｒｉｎｅ，ＴＴＤ）对两种化疗药物细胞毒性的影响。结果ＴＴＤ可明显提高ＤＮＲ及ＨＨＴ对耐药细胞（Ｋ５６２／Ａ０２，Ｋ５６２／ＨＨＴ）的毒性作用，ＩＣ５０值分别下降１５．８及１９．１倍（Ｐ＜０．０５）；而ＴＴＤ对Ｋ５６２／Ｓ敏感细胞无明显影响（Ｐ＞０．０５）。同时进行了异搏定的对照实验。推测ＴＴＤ可能是通过抑制耐药肿瘤细胞膜上Ｐ－１７０糖蛋白的功能而起作用的。

Cytotoxicities of daunorubicin(DNR)and homoharringtonini(HHT) in leukemic cells were measured by MTT method.We studied the effect of Chinese medicine,tetrandrine(TTD)on the cytotoxicities of DNR and HHT. The results showed that TTD could significantly potentiate the toxicities of DNR and HHT in drug-resistant leukemic cell lines (K562/A02,K562/HHT),the IC50 values decreased 15.8 bold and 19.1 fold respectively(P<0.05);but it′s effect on drug-sensitive cell lirie(K562/S)was negligible(P>0.05).Meanwhile,we made the control experiments with verapamil(VRP).TTD may act by means of suppressing the function of the P-170 glycoprotein in drug-resistant tumor cells.