卫氏并殖吸虫固相抗原IFAT和IEST的比较研究

Comparative Studies on Solid Phase Antigen of Paragonimus Westermani by IFAT and IEST

用卫氏并殖吸虫成虫、童虫固相抗原作间接荧光抗体试验（ＩＦＡＴ）及免疫酶染色试验（ＩＥＳＴ）对比检测７９例肺吸虫感染者和２５例健康人血清，两法的阳性率分别为９３．７％、８９．９％和９６．２％、９３．７％，假阳性率均为Ｏ。ＩＦＡＴ和ＩＥＳＴ对比检测２０例血吸虫病人血清、２５例华支睾吸虫病人血清和２０例肺结核病人血清。ＩＦＡＴ出现的交叉反应率为１５％、４％、０和１５％、４％、０。ＩＦＳＴ出现的交叉反应率为５％、０、０和１０％、４％、０。结果表明两法用于卫氏并殖吸虫感染的诊断均有较高的敏感性和特异性，并且抗原定位好．ＩＥＳＴ不需荧光显微镜，用普通生物显微镜就能观察结果，较易推广应用。

Both indirect fluorescent antibody test(IFAT)and immunoenzymatic staining technique(IEST)with frozen sections of adult and adolescent Paragonimus westermani as antigen were employed for detecting 79 cases with Paragonimus Wstermani infection and 25 healthy persons。 The positive rate was 93.7%, 89.9%with IFAT and 96.2%and 93.7%with IEST,respectively. The results Showed no significant statistic difference(P>0.05).The false positive rate were 0.When sera from 20 cases with schistosomiasis, 25 cases with clonorchiasis and 20 cases with tuberculosis were examined by IFAT and IEST,cross-reactions were 15%,4%,0 and 15%,4%,0 with IFAT,and 5%,0, 0 and 10%,4%, 0 with IEST, respectively, The results showed that both IFAT and IEST are useful mothods for serological diagnosis of Paragonimus Wstermani infection and the adult, adolebecent antigen on the epidermis,gut,excretory bladder was well demonstrated, while IEST might be more suitable in field surveys.