离子敏感微电极的制作及参数测试

MAKING OF ION-SENSITIVE MICROELECTRODE AND STUDYING ON THEIR PROPERTIES

本文制作了Ｋ＋，Ｎａ＋液态膜离子敏感微电极（Ｋ＋，Ｎａ＋—ＩＳＭＥ），并稳定了其制作条件，经测试，Ｋ＋—ＩＳＭＥ线性范围为１—１０－４ｍｏｌ／Ｌ，检测下限为３．０×１０－６ｍｏｌ／Ｌ。对Ｎａ＋选择系数为２．６８×１０－４。电位飘移为±０．１ｍＶ／ｈ。Ｎａ＋—ＩＳＭＥ线性范围为１—２．０×１０－ｍｏｌ／Ｌ，检测下限为５．０×１．０－４ｍｏｌ／Ｌ。对Ｋ＋的选择系数为１．８５×１０－２，对Ｃａ２＋的选择系数为１．４６。上述参数均满足生理实验要求，离子敏感微电极技术可做为生理学研究的重要手段。

K+and Na+ sensitive microelectrode was made and its properties were studied in this paper. The Kk,Na (selectivity coefficient of K+-ISME for K+ with respect to Na+ ) was 2. 68±1. 46 ×10-4(n= 5 ). The Nicolsky equation described well the microelectrode potentials from 1mol/L to 10-4 mol/L. Resistance of the microelectrode was 1010Ω. The fluctuation of potential was ± 0. 1 mV/h; KNa,k of Na+-ISME was 1. 85 ±0. 23× 10-2,KNa,Ca was 1. 46 ±0.15(n=4),this indicates that Na +-ISME is more seletive to Ca2+ than to Na+, so their intracellular application requires special care to handle Ca2+ interference under certain conditions. The concentration in which Nicolsky equation described the potentials well was 1mol/L 2 × 10-3mol/L. Potential and resistance of the microelectrode were stable for a long period. These results showed that K+ and Na+-ISME were suitable for measurement of intracellular and extracellular K+, Na+ activities .