期刊文献+

用原位杂交技术对玉米中期染色体中rRNA的研究 被引量:1

STUDIES ON rRNA IN METAPHASE CHROMOSOME OF ZEA MAYS BY IN SITU HYBRIDIZATION
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摘要 本文以玉米17SrRNA的cDNA作探针,采用缺口平移技术,用Bio-dUTP对该探针进行生物素标记。在K4M树脂包埋的玉米根尖超薄切片上进行原位杂交。杂交后,在电镜下通过与10nm金颗粒相连的亲合素对杂交子进行检测,首次直接证实了除核仁,核质和细胞质外,rRNA在中期染色体上的存在。其分布特点是随机的,均匀的,并在数量上明显高于细胞质。 In situ hybridization using cDNA probe of maize 175 rRNA, which was biotinylated by nick-translation technique with Bio-dUTP, was developed on the K4M-embedded ultrathin sections of Zea mays root tip. After hybridization, the biotinylated hybrids were detected with avidin coupled to 10 nm gold particles in electron micrscopy. Besides the presence in nucleolus, cytoplasm,nucleoplasm, rRNA was confirmed directly for the first time to exist in plant metaphase chromosome with higher amount than that in the cytoplasm. It was characterized by random and even distribution.
出处 《南开大学学报(自然科学版)》 CAS CSCD 1995年第3期1-5,共5页 Acta Scientiarum Naturalium Universitatis Nankaiensis
基金 国家教委博士点基金
关键词 原位杂交 玉米 中期染色体 RRNA 染色体 in situ hybridization Zea mays metaphase chromosome rRNA
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参考文献2

  • 1林万明,核酸探针杂交实验技术,1991年
  • 2Fan H,J Mol Biol,1971年,59卷,27页

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  • 2Zhang D M,Sang T.Physical mapping of ribosomal RNA genes in peonies(Paeonia,Paeoniaceae) by fluorescent in situ hybridization:implications for phylogeny and concerted evolution.American Journal of Botany,1999,86:735-740.
  • 3Wasko A P,Galetti Jr P M.Mapping 18S ribosomal genes in fish of the genus Brycon(Characidae) by fluorescence in situ hybridization(FISH).Genetics and Molecular Biology,2000,23(1):135-138.
  • 4Siroky J,Zluvova J,Riha K,Shippen D E,Vyskot B.Rearrangements of ribosomal DNA clusters in late generation telomerase-deficient Arabidopsis.Chromosoma,2003,112:116-123.
  • 5Pillay M,Myers G O.Genetic diversity in cotton assessed by variation in ribosomal RNA genes and AFLP markers.Crop Science,1999,39:1881-1886.
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  • 7Taketa S,Harrison G E,Heslop-Harrison J S.Comparative physical mapping of the 5S and 18S-25S rDNA in nine wild Hordeum species and cytotypes.Theoretical and Applied Genetics,1999,98:1-9.
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