自体雪旺氏细胞植入去肌浆骨骼肌修复周围神经缺损的实验研究

THE EXPERIMENTAL STUDY OF IMPLANTATION OF SCHWANN CELLS INTO REMOVED MYOPLASM SKELETAL MUSCLE TO REPAIR PERIPHERAL NERVE GAP

先用化学手段使骨骼肌细胞脆性增加，再以物理挤压方法去除１／２左右肌浆制成修复周围神经缺损的移植体。将此移植体植入雪旺氏细胞培养液与自体雪旺氏细胞制成的悬液，修复鼠１０ｍｍ坐骨神经缺损，并与热变性骨骼肌植入相同悬液和自体神经移植作对照比较。桥接术后６个月，检测试验侧／正常侧小腿三头肌肌张力比值和湿重比值、试验侧坐骨神经传导速度、和移植体远端／近端神经干髓鞘密度比值４项指标，经统计学处理去肌浆骨骼肌组差于或接近于自体神经移植对照组，而优于或明显优于热变性骨骼肌组。由此提出，去肌浆骨骼肌用于桥接周围神经缺损值得进一步研究。

In this paper the brittleness of skeletal muscle cells was increased by chemical measures. Then this muscle was physically pressed in order to remove the myoplasm into the graft of the peripheral nerve gap.The mixture of the cultural liquid of Schwann cells and autogenous Schwann cells was implanted into the removed myoplasm skeletal muscleto become the graft Of the peripheral nerve gap.The rat sciatic nerve gap of 10mm in length was repaired with this graft,The graft of heat-degenerated skeletal muscle im planted the same mixture and the autogenous nerve was applied to contrast.At 6 months after bridging,we tested the following indexes:1.the muscle tension ratio of tested side/normal side triceps surae muscle;2.the sciatic nerve conduction velocity of tested side,3.the damp weight ratio of tested side/normal side triceps surae muscle and 4.the density ratio of the myelin sheath of distal/proximal nerve trunk of the grart,Through the statistical analyses,the results demonstrated that the group of removed myoplasm skeletal muscle was inferior or close to the group of the autogenous nerve,but superior or obviously superior to the group of the heat-degenerated skeleeal muscle.