血清GPDA同工酶对原发性肝癌的诊断价值

DIAGNOSTIC VALUE OF SERUM GLYCYLPROLINE DIPEPTIDYL AMINOPEPTIDASE ISOENZYME IN PATIENTS WITH PRIMARY HEPATIC CARCINOMA

对５５例原发性肝癌（ＰＨＣ），６６例其他肝病、１６例肝外肿瘤和２２名正常人，应用自行设计的聚丙烯酰胺凝胶阶段梯度电泳作ＧＰＤＡ同工酶检测，将血清ＧＰＤＡ分离出１０条同工酶区带。结果发现血清ＧＰＤＡⅡ／Ⅱ′在ＰＨＣ中的阳性率为４１．８％，而在非癌性肝病和肝外肿瘤患者中阳性率均很低（８．３％～１３．３％），在正常对照组中全部阴性，其对ＰＨＣ诊断敏感性为４１．８％，特异性为８９％。ＡＦＰ阳性与阴性的ＰＨＣ，以及小肝癌与中晚期肝癌患者，血清ＧＰＤＡⅡ／Ⅱ′的阳性率均无显著差异。上述结果表明：血清ＧＰＤＡⅡ／Ⅱ′对诊断ＰＨＣ（包括早期和ＡＦＰ阴性的ＰＨＯ）具有一定的特异性诊断价值。

Serum glycylproline dipeptidyl aminopeptidase(SGPDA) isoenzyme were separated by means of polyacrylamide stage gel electrophoresis in 55 patients with primary hepatic carcinoma(PUC), 66 patients with other liver diseases, 16 patients with extrahepatic tumors and 22 healthy controls. SGPOA was separated into 10 isoenzyme bands. The positive rate of GPDAⅡ/Ⅱ' was 41.8 % in PHC and lower in patients with other liver discases or extrahepatic tumors. No SGPDA Ⅱ/Ⅱ' was found in control subjects. The diagnostic sensitivity and specificity of GPDAⅡ/Ⅱ' for PUC were 41.8% and 89% respectively. Between AFP positive PHC and negative PHC, or belween early PHC and advanced PHC, there were no significant differences of SGPDAⅡ/Ⅱ' positive rate. It was shown that the detection of SGPOAⅡ/Ⅱ' had definite specific value for PHC(including early and AFP negative PHC).