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纤支镜活刷检标本DNA含量的流式细胞仪分析 被引量:1

FLOW CYTOMETRIC ANALYSIS OF BRONCHOSCOPIC BRUSHING AND BIOPSIES FOR DNA CONTENT IN LUNG CANCER
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摘要 本文采用纤维支气管镜(纤支镜)技术,对26例中心型支气管肺癌(肺癌)进行活检和刷检,并将活检和刷检标本制成单细胞悬液,用流式细胞仪测定其DNA含量。结果提示,新鲜肺癌组织中异倍体检出率明显增加,异倍体的出现多与恶性肿瘤存在有关。本文认为,行纤支镜活检和刷检中的任何一项检查,多可获得阳性结果;与活检比,刷检操作更简便,对组织损伤小,出血和并发症少,可弥补管壁浸润型肺癌不易获得活检标本之不足,具有相当大的实用价值。 This study reported exploring the usefulness of flow cytometric analysis of DNA content in lung cancer. Twenty six patients with central lung cancers were studied. With bronchoscopic technique,we make bronchial brushing as well as biopsy specimens,to obtain material for analysis of DNA content and routine cytologic examination. The results suggested that higher levels of DNA aneuploidy were found in fresh lung cancer tissues. The bronchial brushing was simple,scanty bleeding and few complication which were appeared. It was more useful for central lung cancers. So bronchial brushing have a suitable practise method for clinical medicine.
出处 《内镜》 1995年第6期345-347,共3页
关键词 肺肿瘤 流式细胞测定 DNA 纤支镜 活组织检查 Lung neoplasms: Flow cytometry
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  • 1Ottoson GL,Christonson IJ,Larson JK,et al.Tissue disaggregation for flow cytometric analysis:comparison of fine needle aspiration and an automated mechanical procedure[J].Cytometry,1996,26:65-68
  • 2Barlogie B,Drewinko B.Cellar DNA content as a marker of neoplasia in man[J].Am J Med,1980,69:195

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